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MAIN SUBSYSTEMS:Main Support ModuleSample HandlingBore-Hole Inspection Mars Deep DrillRemote Operations

MARTE DRILLING: GROUND TRUTH 2003 GROUND TRUTH 2004

MISSION SIMULATION 2005

2005 MARTE CHRONICLES:CHRONICLE 1 (PDF)CHRONICLE 2 (PDF)
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There are three types of instruments included in the science payload used by MARTE. The first set of instruments is used to characterize the geological and physical attributes of all of the cores. These instruments are referred to as remote sensing instruments. This information will be used to determine where to obtain samples to be used in the second type of instruments referred to as in situ sensing life detection instruments. Two instruments will be used for life detection known as the Signs of Life Detector (SOLID) and BSEDS instruments. SOLID is a portable automated instrument that uses protein microarrays technologies to detect microorganisms. On a Mars mission, the number of analysis chambers available will be limited. Hence it is necessary to pre-screen the cores using the other instruments to identify where it is best to use this limited resource. SOLID and BSEDS accepts powdered subsamples from the CSHS which are then further refined before processing. Finally, an instrument system called the Bore Hole Inspection System (BHIS) is used to collect data in situ at the borehole.

 

Core Context Imager:

PS-S230This imager captures the full length of the 25cm core segment at a resolution of about 125microns/pixel in color. The imager uses a 3.2Mpx (2048x1536 pixels) Canon Power-Shot S230 with remotely operated functions (shutter release, zoom, flash, WB) via a USB connection. The JPEG image file generated by the camera is about 1.6Mb in size.

 

Core Microscopic Imager:

EOS-10DMicroscopic images of the core are acquired using a Canon EOS-10D with a 100mm macro-lens. This obtains color microscopic quality images of pre-determined sections of the core with about a 7 microns per pixel resolution and a field of view of 2.5x 1.66 cm. The sizes of the JPEG files generated are about 6.7Mb and are transmitted via USB interface. The core will be imaged microscopically at predetermined positions (we expect approximately 4 images per 25cm section of core). The number of these images is limited by operational constraints such as time and data volume.

 

Visible Near Infrared Spectrometer:

The sections of the core selected to be imaged with the microscopic imager are then analyzed with the VNIR spectrometer. The VNIR spectrometer is an Ocean Optics S2000 Temperature Regulated Spectrometer, with a #3 grate (range of 400nm to 1050nm) with a high-sensitivity linear CCD array (2048 element). The spectrometer system is composed of the spectrometer enclosed inside a temperature control box capable of maintaining the set temperature to within 0.1C; an A/D converter box with USB computer interface, and a Tungsten-halogen illumination lamp. The reflectance probe contains six illuminating and one read fibers, all 400micrometers in diameter, UV/VIS; and is placed at an optimum distance to collect spectra of a spot 5mm in diameter.

 

Imaging Spectrograph:

Imaging SpectrographThe scanning spectrograph permits high-resolution imaging of rock core samples with a high degree of spectral information. The instrument utilizes macroscopic imaging optics and a slit input aperture to sample a line on the object (core). A combination of dispersive and diffractive elements and relay lenses provide light to the array detector, which is separated spatially along the slit in one dimension and spectrally along the other array dimension. This is sampled with an 8-bit monochrome industrial vision CCD camera. Scanning the object in a direction normal to the slit while taking multiple images, permits the formation of a data “hyper-cube” in the mode of classic “push-broom” type remote sensing instruments (e.g. PHILLS, HYDICE). The spectrograph resolves 580 columns of 50-microns spatial elements along the input aperture. The hypercube image length is only limited by the length of the scan, presently configured for rock cores that are 25 cm long. The spectrum is sampled by 780 detector rows spanning the 400 to 1000 nanometer wavelength range. This over-sampled spectral data is averaged, typically over 6 rows, to improve signal quality and to reduce hypercube size.

The spectrograph will operate under current-controlled tungsten-halogen illumination to permit the derivation of reflectance data. A spectral calibration is performed using Krypton and Mercury-vapor emission lamps. Radiometric calibration processes include dark frame subtraction and normalization by a reference frame taken with 99% Spectralon in the object field. A "quick-look" color image is produced at the end of the scan comprised of three wavelength bands that represent normal human perception of red(660 nm), green (530 nm) and blue (460 nm). The instrument operator may specify regions of interest in the quick-look image, and load a request to the hypercube data mining software, which will return reflectance spectra data averaged over these regions. The instrument provides only ~48 db max signal-to-noise ratio, as limited by the dynamic range of the camera and particularly limited in the short- and long-wavelength regions by the spectral response of the silicon CCD sensor and the black-body intensity distribution of the tungsten-halogen lamps.

Core Outer Surface Biology Sampling:

Lighting MVP The external surface of all received cores will be sampled and tested for the presence of adenosine triphospahte (ATP) (via bioluminescence) with the Lightning MVP instrument (manufactured by BioControl Systems Inc.) The Lightning MVP instrument uses a Luciferin-luciferase reagent to react with ATP to emit light. A cotton swab is swept across the surface of a core, then placed into a chamber where a reagent is added then the swab is exposed to a UV light. The reagent causes fluorescence in proportion to the amount of ATP present. The reading takes about 10s and it is measured in relative light units (RLU). The MVP instrument has a sensitivity of 15 Pico-grams of ATP. ATP is the energy mechanism for all living organisms so ATP luminometry is used as a screening instrument to determine the level of bacterial bioburden present on the cores. In MARTE it will provide a "quick look" assay for the presence of bacteria in cores.

 

Signs of Life Detection System (SOLID):

SOLIDSOLID screen

The instrument is a compact portable automated instrument that uses protein microarray technologies to detect microorganisms as well as their metabolic products. The aim is to detect any kind of biochemical compound (nucleic acids, proteins, polysaccharides, etc) using microarrays printed with antibodies or any other protein or molecule able to recognize and bind specifically to them. Molecular biology techniques allow fluorescent labeling of either the targets or the probes. A laser beam excites the sample and a CCD camera detects the bright spots. Core sections of scientific interest are sliced, powdered, and run through a series of molecular biology techniques using the SOLID instrument. SOLID performs an in-situ analysis of the core by using micro-arrays containing thousands of probes to detect organic compounds and whole cell characteristics from the powdered core samples, with a resolution better than PPB. Only 0.5 grams of powdered rock are required to conduct the procedure.

 

Biological Sample Extraction and Detection System (BSEDS) :

Biological Sample Extraction& Detection SystemBSEDS uses Limulus Amebocyte Lysate (LAL) assay techniques to screen for biology and has as its main purpose to complement and make more robust, the existing biology detection system capability of the MARTE systems. The Limulus Amebocyte Lysate assay (LAL) is basically an aqueous enzyme extract from the blood cells of the horseshoe crab (Limulus Polyphemus) and is highly reactive to the bacterial endotoxins that are part of the lipopolysaccharide complex of gram negative bacteria and the beta glucan components of yeasts. When in the presence of the endotoxin, LAL initiates a cascade of enzyme activation steps that result in the proteolytic cleavage of an artificial peptide substrate. Upon cleavage this substrate releases p-nitroanaline, which is yellow in color and absorbs at 405 nm. The amount of endotoxin in the sample is quantified by the onset time of reaction and is measured using a laser diode at 405 nm coupled with a spectrophotometer. Hence shorter reaction times signify a higher amount of endotoxin in the sample and a standard endotoxin curve is used to help determine actual concentration of endotoxin in a given sample.

The system that will be used for the MARTE project is a modified version of commercially available handheld instrument called the Endosafe Portable Test System (PTS). The PTS will be coupled to an autonomous sample extraction system that will also provide the researcher with information about pH of the sample, specific ion concentration, and temperature. LAL uses a measure of endotoxin units per milliliter of sample (EU/ml). This measurement can be correlated back to the instrument calibration curve to obtain an approximate cell concentrating in the traditional measure of CFU/ml. The current sensitivity of the PTS system is 0.05 EU/ml which is equivalent to less than 1 CFU/ml. It is estimated that the PTS can detect as low at 1 cell per ml of sample.

Centro de Astrobiologia Honeybee RoboticsUniversity of Oklahoma KISS Institute for Practical RoboticsStevens Research

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